12th Global Conference on
Gynecology, Obstetrics & Women’s Reproductive Health

Mohammad Al Obeed Allah

Charles University's, Czech Republic

Abstract Title:

Advancing Digital PCR for the Identification and Detection of Clinically Relevant Gene Variants in Ovarian Cancer

Biography:

Mohammad Al Obeed Allah, MD, is a dedicated researcher and medical professional. Currently pursuing a PhD in molecular biology at Charles University's Faculty of Medicine in Pilsen, Czechia. His impactful research in oncology as a Biomedical Center researcher at Charles University underscores his commitment to advancing medical science. He aims to validate pivotal genetic variants related to ovarian carcinoma progression and resistance. Recipient of the Charles University Grant Agency award, his dedication, and achievements exemplify his passion for medical advancement.

Research Interests:

Using the QIAcuity Digital PCR System and Qiagen dPCR LNA Mutation Assays, we optimized multiplex detection of TP53 variants (p.Tyr220Cys, p.Arg248Gln, p.Arg273His) and KRAS variants (p.Gly12Val, p.Gly12Ala, p.Gln61His) in DNA from tumor tissues (N=44), an independent validation set (N=125), and plasma samples (N=10). Additionally, singleplex detection of TP53 variants (p.Arg175His, p.Arg248Trp) was optimized.